Expression and characteristics of the gene encoding azoreductase from Rhodobacter sphaeroides AS1.1737 | Zendy

Bin Yan | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Expression and characteristics of the gene encoding azoreductase from Rhodobacter sphaeroides AS1.1737

Author(s) -

Bin Yan

Publication year - 2004

Publication title -

fems microbiology letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.899

H-Index - 151

eISSN - 1574-6968

pISSN - 0378-1097

DOI - 10.1016/j.femsle.2004.05.034

Subject(s) - rhodobacter sphaeroides , gene , rhodospirillaceae , escherichia coli , rhodospirillales , chemistry , enzyme , gene expression , biochemistry , microbiology and biotechnology , biology , photosynthesis

A gene that encodes a protein with azoreductase activity was obtained by PCR amplification from Rhodobacter sphaeroides AS1.1737. The enzyme, with a molecular weight of 18.7 kD, was heterologously expressed in Escherichia coli and its azoreductase activity was characterized. Furthermore, the reduction mechanism of azo dyes catalyzed by the azoreductase was studied in detail. The presence of a hydrazo-intermediate was identified, which provided a convincing evidence for the assumption that azo dyes were degraded via an incomplete reduction stage.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research