Transformation of colonial Escherichia coli on solid media | Zendy

Sumio Maeda | Zendy

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Transformation of colonial Escherichia coli on solid media

Author(s) -

Sumio Maeda

Publication year - 2004

Publication title -

fems microbiology letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.899

H-Index - 151

eISSN - 1574-6968

pISSN - 0378-1097

DOI - 10.1016/j.femsle.2004.05.023

Subject(s) - escherichia coli , transformation (genetics) , agar , microbiology and biotechnology , plasmid , biology , agar plate , bacteria , dna , genetics , gene

We report that colonial Escherichia coli cells on various solid media can develop modest genetic competence. Using an on-filter culture system, we found that E. coli colonies on CaCl2-containing agar were transformed in the presence of plasmid DNA. Interestingly, transformation also occurred on LB-agar, various moist foods and even on H2O-agar. These results suggest that some populations of colonial E. coli in various environments could become transformable regardless of the surrounding Ca2+ concentration.

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