Structure and mode of transposition of Tn2555 carrying sucrose utilization genes | Zendy

Vladimir M. Doroshenko | Zendy

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Structure and mode of transposition of Tn2555 carrying sucrose utilization genes

Author(s) -

Vladimir M. Doroshenko

Publication year - 2004

Publication title -

fems microbiology letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.899

H-Index - 151

eISSN - 1574-6968

pISSN - 0378-1097

DOI - 10.1016/j.femsle.2004.03.016

Subject(s) - transposable element , replicon , transposition (logic) , tn3 transposon , plasmid , insertion sequence , genetics , biology , escherichia coli , transposase , inverted repeat , transposon mutagenesis , gene , recombination , microbiology and biotechnology , mutant , genome , linguistics , philosophy

The sucrose transposon Tn2555 from Escherichia coli, which has an unstable structure, was studied in more detail. Sequence analysis of one of the transposon variants, designated Tn2555.3, revealed the presence of two direct IS26 copies on its flanks, and a third inverted IS26 copy inside the transposon. The sucrose utilization genes of Tn2555.3 were found to be identical to those of the previously described pUR400 plasmid. It was demonstrated that Tn2555.3 translocation from pBR325 to RP4 occurs via a cointegrate formation, mediated by one of the three IS26 copies, followed by its resolution due to RecA-dependent recombination between two direct IS26 copies flanking the donor replicon.

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