Identification of a P2-related prophage remnant locus of Photorhabdus luminescens encoding an R-type phage tail-like particle | Zendy

Sophie Gaudriault | Zendy

Open Access

Identification of a P2-related prophage remnant locus of Photorhabdus luminescens encoding an R-type phage tail-like particle

Author(s) -

Sophie Gaudriault

Publication year - 2004

Publication title -

fems microbiology letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.899

H-Index - 151

eISSN - 1574-6968

pISSN - 0378-1097

DOI - 10.1016/j.femsle.2004.02.009

Subject(s) - photorhabdus luminescens , prophage , biology , locus (genetics) , genetics , photorhabdus , lysogenic cycle , gene , whole genome sequencing , genome , sequence analysis , microbiology and biotechnology , escherichia coli , bacteriophage

Analysis of the Photorhabdus luminescens genome sequence revealed that the pts region is related to the tail synthesis gene core of the P2 phage. The pts locus encodes a DNA invertase homologue. PCR-RFLP analysis showed the two potential tail fiber regions of the pts locus present DNA inversions. Electron microscopy revealed a phage tail-like particle, related to the R-type family and named R-photorhabdicin, in the culture supernatant of P. luminescens. Mass spectrometry analysis of two sub-units of R-photorhabdicin revealed that they are encoded by the pts locus. The role of this P2-related prophage remnant in the Photorhabdus genome is discussed.

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