Open AccessPhospholipids of Clostridium perfringens : a reexamination
Author(s) -
Johnston Norah C.,
Baker John K.,
Goldfine Howard
Publication year - 2004
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2004.01.048
Subject(s) - phosphatidylethanolamine , phosphatidylglycerol , myristic acid , phospholipid , clostridium perfringens , chemistry , lauric acid , palmitic acid , chromatography , fatty acid , biochemistry , phosphatidylcholine , biology , bacteria , membrane , genetics
We have identified phosphatidylethanolamine as one of the major phospholipids of Clostridium perfringens by two dimensional thin layer chromatography of the intact lipids and of their deacylation products and by liquid chromatography followed by mass spectrometry of the intact neutral phospholipid fraction. The principal fatty acids of phosphatidylethanolamine are myristic acid (14:0), lauric acid (12:0), and palmitic acid (16:0) and the major molecular species are 14:0,14:0 (26.3%); 12:0,14:0 (19.0%); 14:0,16:0 (22.4%) and 16:0,16:0 (17.6%). A similar distribution of molecular species was found in the other major phospholipid, O ‐alanyl phosphatidylglycerol.