Open AccessDevelopment of PCR assays for the detection and differentiation of Fusarium sporotrichioides and Fusarium langsethiae
Author(s) -
Wilson Anthony,
Simpson Duncan,
Chandler Elizabeth,
Jennings Phil,
Nicholson Paul
Publication year - 2004
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2004.01.040
Subject(s) - fusarium , biology , phylogenetic tree , trichothecene , polymerase chain reaction , microbiology and biotechnology , gene , genetics
Isolates of the type‐A trichothecene producing Fusarium sporotrichioides and Fusarium langsethiae were grouped and differentiated in a phylogenetic tree using ITS sequence dissimilarity. An attempt was made to develop a PCR‐based assay for the detection and differentiation of Fusarium sporotrichiodes from other Fusarium species using the 5 ′ ‐region of the tri5 gene as a template. However, this assay was unable to differentiate, to a satisfactory level, between isolates of Fusarium sporotrichioides and Fusarium langsethiae , providing further genetic evidence for their close genetic relationship. A robust and repeatable PCR‐assay was developed for the detection and differentiation of both species based on sequence determined from differentially amplified RAPD‐PCR products. These assays were able to detect both species in samples of grain taken from the field.