Open AccessHomology, disruption and phenotypic analysis of CaGS Candida albicans gene induced during macrophage infection
Author(s) -
Luongo Marina,
Porta Amalia,
Maresca Bruno
Publication year - 2005
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1016/j.femsim.2005.06.007
Subject(s) - candida albicans , biology , macrophage , microbiology and biotechnology , phenotype , mutant , gene , corpus albicans , saccharomyces cerevisiae , fungal protein , in vitro , genetics
During macrophage infection Candida albicans expresses differentially several genes whose functions are associated with its survival strategy. Among others, we have isolated CaGS gene, which is homologous to SNF3 , a glucose sensor of Saccharomyces cerevisiae . To elucidate its potential role during infection, CaGS has been disrupted and the resulting phenotype analyzed on different solid media. The null mutant lost the ability to form hyphae on a medium with low glucose concentration and serum. Furthermore, this mutant does not disrupt macrophage in in vitro infections. We believe that this putative glucose sensor is involved in hyphal development during macrophage infection.