Open AccessIdentification of the infectious source of an unusual outbreak of histoplasmosis, in a hotel in Acapulco, state of Guerrero, Mexico
Author(s) -
Taylor Maria Lucia,
RuízPalacios Guillermo M.,
Rocío ReyesMontes María,
RodríguezArellanes Gabriela,
CarretoBinaghi Laura E.,
DuarteEscalante Esperanza,
HernándezRamírez Aurora,
Pérez Armando,
SuárezAlvarez Roberto O.,
RoldánAragón Yuri A.,
RomeroMartínez Rafael,
SahazaCardona Jorge H.,
SifuentesOsornio José,
SotoRamírez Luis E.,
PeñaSandoval Gabriela R.
Publication year - 2005
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1016/j.femsim.2005.05.017
Subject(s) - biology , outbreak , histoplasmosis , nested polymerase chain reaction , virology , histoplasma , histoplasma capsulatum , polymerase chain reaction , microbiology and biotechnology , gene , genetics , immunology
Three isolates of Histoplasma capsulatum were identified from mice lung, liver, and spleen inoculated with soil samples of the X hotel's ornamental potted plants that had been fertilized with organic material known as compost. The presence of H. capsulatum in the original compost was detected using the dot‐enzyme‐linked immunosorbent assay. Nested‐PCR, using a specific protein Hcp100 coding gene sequence, confirmed the fungal identification associated with an unusual histoplasmosis outbreak in Acapulco. Although, diversity between the H. capsulatum isolate from the hotel and some clinical isolates from Guerrero (positive controls) was observed using random amplification of polymorphic DNA based‐PCR, sequence analyses of H‐anti and ole fragment genes revealed a high homology (92–99%) between them.