Open AccessElectrophoresis karyotype and chromosome‐length polymorphism of Histoplasma capsulatum clinical isolates from Latin America
Author(s) -
Canteros Cristina Elena,
Zuiani María Fernanda,
Ritacco Viviana,
Perrotta Diego E.,
ReyesMontes María Rocío,
Granados Julio,
Zúñiga Gerardo,
Taylor Maria Lucia,
Davel Graciela
Publication year - 2005
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1016/j.femsim.2005.05.015
Subject(s) - biology , karyotype , genetics , chromosomal polymorphism , polymorphism (computer science) , histoplasma capsulatum , chromosome , genotype , microbiology and biotechnology , virology , histoplasmosis , gene , immunology
Intact chromosomes of 19 clinical isolates of Histoplasma capsulatum recently obtained in Argentina, Mexico and Guatemala and the laboratory reference strain G186B from Panama were analyzed using pulsed‐field gel electrophoresis. Chromosomal banding patterns of the human isolates revealed 5–7 bands, ranging from 1.3 to 10 Mbp in size. Strain G186B showed five bands of approximately 1.1, 2.8, 3.3, 5.4 and 9.7 Mbp. Thirteen different electrokaryotypes were identified, indicating that the genome of H. capsulatum varies widely in nature, as observed previously in laboratory strains. No definite association was found between electrokaryotype and geographical or clinical source.