Open AccessSecretion of matrix metalloproteinase‐9 by macrophages, in vitro , in response to Helicobacter pylori
Author(s) -
James Bergin Philip,
Sicheng Wen,
Qiang PanHammarström,
Marianne QuidingJärbrink
Publication year - 2005
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1016/j.femsim.2005.03.008
Subject(s) - secretion , helicobacter pylori , biology , matrix metalloproteinase , autocrine signalling , inflammation , macrophage , tumor necrosis factor alpha , interleukin 8 , immunology , microbiology and biotechnology , in vitro , cell culture , endocrinology , biochemistry , genetics
Abstract We have previously shown that matrix metalloproteinase‐9 (MMP‐9) activity is greatly enhanced within the active chronic inflammation of Helicobacter pylori infected individuals, of which a major fraction derives from macrophages in the tissue. Here, we have investigated the ability of macrophages to secrete MMPs in response to H. pylori . Human macrophages secrete MMP‐9 in response to live and inactivated H. pylori , as well as to specific bacterial products. Protein kinase C, phosphatiolylinositol 3‐kinase and calcium uptake channels all play a role in MMP‐9 secretion, whereas neither tumour necrosis factor alpha, interleukin‐8, nor interleukin‐1β autocrine stimulation appear to contribute. We conclude that human macrophages have the ability to react directly against several H. pylori derived factors, utilising several signalling pathways.