Open AccessClass 1 integrons in Pseudomonas aeruginosa isolates from clinical settings in Amazon region, Brazil
Author(s) -
Fonseca Érica L.,
Vieira Verônica V.,
Cipriano Rosângela,
Vicente Ana C.P.
Publication year - 2005
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1016/j.femsim.2005.01.004
Subject(s) - integron , biology , gene cassette , pseudomonas aeruginosa , microbiology and biotechnology , restriction fragment length polymorphism , multilocus sequence typing , gene , genetics , polymerase chain reaction , antibiotic resistance , genotype , bacteria , antibiotics
A hundred and six Pseudomonas aeruginosa isolates from clinical cases were screened using PCR for the presence of integrons and associated resistance gene cassettes. Forty‐four isolates harboured class 1 integrons (41.5%), of which 29 isolates (66%) also carried gene cassettes. The aac A gene was most frequently found within class 1 integrons (69%), followed by bla OXA family genes (52%). From class 1 integron‐positive strains, we detected a total of 15 isolates (34%) carrying no gene cassettes. Restriction fragment‐length polymorphism analysis of the integrons variable region revealed some identical structures, as well as distinct profiles indicating heterogeneity among these cassette regions. Multiresistance was observed in 71% of isolates, nevertheless no strong correlation was observed between integron presence and multiresistance. This is the first report showing class 1 integron prevalence and gene cassette content in P. aeruginosa isolates from clinical settings in the Brazilian Amazon.