Open AccessA swine toll‐like receptor 2‐expressing transfectant as a potential primary screening system for immunobiotic microorganisms
Author(s) -
Tohno Masanori,
Kitazawa Haruki,
Shimosato Takeshi,
Matsumoto Mayumi,
Katoh Shinichiro,
Kawai Yasushi,
Saito Tadao
Publication year - 2005
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1016/j.femsim.2004.12.009
Subject(s) - lipoteichoic acid , zymosan , chinese hamster ovary cell , biology , tlr2 , peptidoglycan , toll like receptor , transfection , microbiology and biotechnology , receptor , bacteria , yeast , tlr4 , biochemistry , cell wall , innate immune system , gene , in vitro , genetics , staphylococcus aureus
Toll‐like receptor 2 (TLR2) has been shown to mediate cell signaling in response to microbial cell wall components, such as peptidoglycan, lipoteichoic acid, microbial lipoprotein, and zymosan. In this study, we cloned the swine TLR2 and used it to transfect Chinese hamster ovary K‐1 cells. We demonstrated that the swine TLR2‐expressing transfectant can bind not only zymosan from yeast cell wall components but also intact lactic acid bacteria, resulting in the activation of nuclear factor‐κB. These findings suggest that the swine TLR2‐expressing transfectant can be very useful for the primary screening of immunobiotic microorganisms.