Open AccessRibotyping of Burkholderia mallei isolates
Author(s) -
Harvey Steven P.,
Minter Jennifer M.
Publication year - 2005
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1016/j.femsim.2004.12.002
Subject(s) - ribotyping , biology , ecori , microbiology and biotechnology , psti , restriction enzyme , subspecies , burkholderia , escherichia coli , genetics , ribosomal dna , polymerase chain reaction , bacteria , dna , gene , phylogenetics , paleontology
In this study, the subspecies differentiation of 25 isolates of Burkholderia mallei was attempted based on their ribotype polymorphisms. The isolates were from human and equine infections that occurred at various times around the world. DNA samples from each isolate were digested separately with Pst I and Eco RI enzymes and probed with an Escherichia coli ‐derived 18‐mer rDNA sequence to identify diagnostic fragments. Seventeen distinct ribotypes were identified from the combined data obtained with the two restriction enzymes. The results demonstrate the general utility of ribotyping for the subspecies identification of B. mallei isolates.