Open AccessDetection and differentiation of Burkholderia pseudomallei , Burkholderia mallei and Burkholderia thailandensis by multiplex PCR
Author(s) -
Lee MayAnn,
Wang Dongling,
Yap Eu Hian
Publication year - 2005
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1016/j.femsim.2004.10.008
Subject(s) - burkholderia pseudomallei , melioidosis , biology , burkholderia , microbiology and biotechnology , multiplex polymerase chain reaction , polymerase chain reaction , multiplex , virology , bacteria , gene , genetics
Abstract Burkholderia pseudomallei , a Gram‐negative bacterium that causes melioidosis may be differentiated from closely related species of Burkholderia mallei that causes glanders and non‐pathogenic species of Burkholderia thailandensis by multiplex PCR. The multiplex PCR consists of primers that flank a 10‐bp repetitive element in B. pseudomallei and B. mallei amplifying PCR fragment of varying sizes between 400–700 bp, a unique sequence in B. thailandensis amplifying a PCR fragment of 308 bp and the metalloprotease gene amplifying a PCR fragment of 245 bp in B. pseudomallei and B. thailandensis . The multiplex PCR not only can differentiate the three Burkholderia species but can also be used for epidemiological typing of B. pseudomallei and B. mallei strains.