Open AccessEnhanced resistance to Gram‐positive bacterium and increased susceptibility to bacterial endotoxin in mice sensitized with Propionibacterium acnes : involvement of Toll‐like receptor
Author(s) -
Mochizuki Hirofumi,
Nomura Takamasa,
Kawamura Ikuo,
Mitsuyama Masao
Publication year - 2005
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1016/j.femsim.2004.09.009
Subject(s) - propionibacterium acnes , microbiology and biotechnology , tlr2 , biology , listeria monocytogenes , innate immune system , immunity , peptidoglycan , toll like receptor , bacteria , immunology , immune system , genetics
Mice sensitized with Propionibacterium acnes showed an enhanced resistance against infection with Listeria monocytogenes in contrast to the increased susceptibility to LPS‐induced endotoxin shock. The enhanced protection to L. monocytogenes was mediated by activated innate immunity but not by generation of Listeria ‐specific acquired immunity. After infection with L. monocytogenes , the elimination of bacteria was observed earlier in accordance with a higher level of endogenous cytokine production in P. acnes ‐sensitized mice than in control mice. Peritoneal cells from P. acnes ‐sensitized mice produced a larger amount of IL‐12p70 and nitric oxide after stimulation with heat‐killed L. monocytogenes or peptideglycan purified from Staphylococcus aureus . RT‐PCR analysis showed that the expression of TLR2 but not TLR1, TLR4 nor TLR6 was induced by injection of P. acnes in peritoneal cells. These results indicated that P. acnes ‐sensitization could induce the activation of innate immunity against L. monocytogenes through increased recognition of bacterial components by TLR2.