Open AccessStructure of the O‐polysaccharide and serological studies of the lipopolysaccharide of Proteus penneri 60 classified into a new Proteus serogroup O70
Author(s) -
Zych Krystyna,
Perepelov Andrei,
Baranowska Agata,
Zabłotni Agnieszka,
Shashkov Alexander S.,
Knirel Yuriy A.,
Sidorczyk Zygmunt
Publication year - 2005
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1016/j.femsim.2004.09.004
Subject(s) - proteus , antiserum , polysaccharide , lipopolysaccharide , microbiology and biotechnology , biology , polyclonal antibodies , proteus vulgaris , strain (injury) , serology , bacteria , stereochemistry , biochemistry , chemistry , escherichia coli , antibody , immunology , genetics , antibacterial activity , anatomy , gene
An alkali‐treated lipopolysaccharide of Proteus penneri strain 60 was studied by chemical analyses and 1 H, 13 C and 31 P NMR spectroscopy, and the following structure of the linear pentasaccharide–phosphate repeating unit of the O‐polysaccharide was established:Rabbit polyclonal O‐antiserum against P. penneri 60 reacted with both core and O‐polysaccharide moieties of the homologous LPS. Based on the unique O‐polysaccharide structure and serological data, we propose to classify P. penneri 60 into a new, separate Proteus serogroup O70. A weak cross‐reactivity of P. penneri 60 O‐antiserum with the lipopolysaccharide of Proteus vulgaris O8, O15 and O19 was observed and discussed in view of the chemical structures of the O‐polysaccharides.