Kinetic studies of bioactive products nitric oxide and 8‐iso‐PGF 2α  in  Burkholderia pseudomallei  infected human macrophages, and their role in the intracellular survival of these organisms | Zendy

Nathan Sakthi A. | Zendy; Qvist Rajes | Zendy; Puthucheary Savithri D. | Zendy

Open Access

Kinetic studies of bioactive products nitric oxide and 8‐iso‐PGF 2α in Burkholderia pseudomallei infected human macrophages, and their role in the intracellular survival of these organisms

Author(s) -

Nathan Sakthi A.,

Qvist Rajes,

Puthucheary Savithri D.

Publication year - 2005

Publication title -

fems immunology & medical microbiology

Language(s) - English

Resource type - Journals

eISSN - 1574-695X

pISSN - 0928-8244

DOI - 10.1016/j.femsim.2004.08.001

Subject(s) - burkholderia pseudomallei , nitric oxide , melioidosis , microbiology and biotechnology , burkholderia , macrophage , intracellular , chemistry , lipid peroxidation , bacteria , biochemistry , oxidative stress , biology , organic chemistry , in vitro , genetics

The oxidative response of Burkholderia pseudomallei and Escherichia coli infected macrophages from normal and melioidosis subjects was determined by measuring the production of nitric oxide which is one of the reactive nitrogen intermediates, and the activation state of these macrophages was determined by measuring the generation of 8‐iso‐PGF 2α , a bioactive product of free radical induced lipid peroxidation. Macrophages obtained from the melioidosis patients generated significantly lower levels of nitric oxide and 8‐iso‐PGF 2α compared to macrophages obtained from the normal subjects ( P < 0.001 ). The reduced efficiency of the oxygen dependent microbicidal mechanism in macrophages of melioidosis patients may be one of the survival strategies developed by B. pseudomallei to remain viable intracellularly.

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