Open AccessApoptosis of phagocytic cells induced by Candida albicans and production of IL‐10
Author(s) -
Gasparoto Thaís Helena,
Gaziri Luis Carlos Jabur,
Burger Eva,
Almeida Ricardo Sérgio Couto,
Felipe Ionice
Publication year - 2004
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1016/j.femsim.2004.05.006
Subject(s) - apoptosis , candida albicans , biology , microbiology and biotechnology , necrosis , phagocytosis , tumor necrosis factor alpha , corpus albicans , macrophage , peritoneal cavity , pepstatin , immunology , in vitro , enzyme , biochemistry , genetics , protease , anatomy
Abstract Macrophages co‐incubated with Candida albicans strain CR1 in vitro showed early signs of apoptosis, but evolved to necrosis after 2 h. In this study, we investigated whether strain CR1 caused apoptosis or necrosis of macrophages after its inoculation into mice peritoneal cavity, and whether this correlated with the secretion of IL‐10. Peritoneal macrophages from mice that received an inoculum of C. albicans CR1 showed signs of apoptosis and necrosis from 30 min to 2 h afterwards, whereas heat‐killed C. albicans did not cause those effects. IL‐10 production was low during the first 6 h post‐infection, when macrophages predominated in the peritoneal exudate, whereas its higher production after 24 h correlated with an increase of neutrophils in the exudate. Treatment of CR1 with pepstatin (an inhibitor of proteinases) prevented the process of apoptosis and significantly reduced IL‐10 production, suggesting that the increased production of IL‐10 was caused by processes occurring during the initial phase of infection, such as apoptosis, necrosis and uptake of death cells.