Open AccessColonisation of Pinus halepensis roots by Pseudomonas fluorescens and interaction with the ectomycorrhizal fungus Suillus granulatus
Author(s) -
Rincón Ana,
RuizDíez Beatriz,
GarcíaFraile Sonia,
García José Antonio Lucas,
FernándezPascual Mercedes,
Pueyo José J.,
Felipe María R.
Publication year - 2005
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1016/j.femsec.2004.09.006
Subject(s) - biology , colonisation , pseudomonas fluorescens , seedling , ectomycorrhiza , inoculation , botany , ectomycorrhizae , mycorrhiza , pisolithus , fungus , bacteria , pinus thunbergii , colonization , microbiology and biotechnology , symbiosis , horticulture , genetics
Colonisation of Pinus halepensis roots by GFP‐tagged Pseudomonas fluorescens Aur6 was monitored by epifluorescence microscopy and dilution plating. Aur6‐GFP was able to colonise and proliferate on P. halepensis roots. Co‐inoculation with the ectomycorrhizal fungus Suillus granulatus did not affect the bacterial colonisation pattern whereas it had an effect on bacterial density. Bacterial counts increased during the first 20 days of seedling growth, irrespective of seedlings being mycorrhizal or not. After 40 days, bacterial density significantly decreased and bacteria concentrated on the upper two‐thirds of the pine root. The presence of S. granulatus significantly stimulated survival of bacteria in the root elongation zone where fungal colonisation was higher. The number of mycorrhizas formed by S. granulatus was not affected by co‐inoculation with Aur6‐GFP. Neither Aur6‐GFP nor S. granulatus stimulated P. halepensis development when inoculated alone, but a synergistic effect was observed on seedling growth when bacteria and fungus were co‐inoculated.