Open AccessKinetics, diffusional limitation and microscale distribution of chemistry and organisms in a CANON reactor
Author(s) -
Nielsen Michael,
Bollmann Annette,
Sliekers Olav,
Jetten Mike,
Schmid Markus,
Strous Marc,
Schmidt Ingo,
Larsen Lars Hauer,
Nielsen Lars Peter,
Revsbech Niels Peter
Publication year - 2005
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1016/j.femsec.2004.09.003
Subject(s) - anammox , anoxic waters , nitrification , ammonia , anaerobic exercise , oxygen , nitrite , environmental chemistry , autotroph , biology , bioreactor , ammonium , nitrogen , chemistry , bacteria , denitrification , nitrate , biochemistry , ecology , botany , organic chemistry , physiology , genetics , denitrifying bacteria
In the Completely Autotrophic Nitrogen removal Over Nitrite (CANON) process, aerobic and anaerobic ammonia oxidizing bacteria cooperate to remove ammonia in one oxygen‐limited reactor. Kinetic studies, microsensor analysis, and fluorescence in situ hybridization on CANON biomass showed a partial differentiation of processes and organisms within and among aggregates. Under normal oxygen‐limited conditions (∼5 μM O 2 ), aerobic ammonia oxidation (nitrification) was restricted to an outer shell (<100 μm) while anaerobic ammonia oxidation (anammox) was found in the central anoxic parts. Larger type aggregates (>500 μm) accounted for 68% of the anammox potential whereas 65% of the nitrification potential was found in the smaller aggregates (<500 μm). Analysis with ∼5 μM O 2 microsensors showed that the thickness of the activity zones varied as a function of bulk O 2 and NO − 2 concentrations and flow rate.