Open AccessEffective cellulose degradation by a mixed‐culture system composed of a cellulolytic Clostridium and aerobic non‐cellulolytic bacteria
Author(s) -
Kato Souichiro,
Haruta Shin,
Cui Zong Jun,
Ishii Masaharu,
Igarashi Yasuo
Publication year - 2004
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1016/j.femsec.2004.07.015
Subject(s) - cellulose , bacteria , biology , clostridium , clostridiaceae , cellulase , food science , microbiology and biotechnology , anaerobic exercise , degradation (telecommunications) , anaerobic bacteria , microbial biodegradation , microorganism , biochemistry , physiology , telecommunications , genetics , computer science
A stable cellulose‐degrading microflora enriched from composting materials has been analyzed in our laboratory. Cellulose‐degrading efficiency of an anaerobic cellulolytic isolate, Clostridium straminisolvens CSK1, was remarkably lower than that of the original microflora. We successfully constructed bacterial communities with effective cellulose degradation by mixing C. straminisolvens CSK1 with aerobic non‐cellulolytic bacteria isolated from the original microflora. Comparison of the cellulose degradation processes of the pure culture of C. straminisolvens CSK1 and the mixed‐culture indicated that non‐cellulolytic bacteria essentially contribute to cellulose degradation by supplying anaerobic environment, consuming metabolites, which otherwise deteriorate the cellulolytic activity, and by neutralizing pH.