Open AccessProteomics as a way to identify extra‐radicular fungal proteins from Glomus intraradices – RiT‐DNA carrot root mycorrhizas
Author(s) -
DumasGaudot Eliane,
Valot Benoît,
BestelCorre Gwénaëlle,
Recorbet Ghislaine,
StArnaud Marc,
Fontaine Bastien,
Dieu Marc,
Raes Martine,
Saravanan Ramu Subramania,
Gianinazzi Silvio
Publication year - 2004
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1016/j.femsec.2004.02.015
Subject(s) - biology , glomus , daucus carota , symbiosis , fungus , botany , phycomycetes , proteomics , gel electrophoresis , glomeromycota , mycorrhiza , biochemistry , microbiology and biotechnology , inoculation , bacteria , spore , gene , genetics , horticulture
To identify fungal proteins involved in the arbuscular mycorrhizal symbiosis, root‐inducing transferred‐DNA transformed roots of carrot ( Daucus carota L.) were in vitro inoculated with Glomus intraradices . Proteins extracted from the extra‐radical fungus were analysed by two‐dimensional gel electrophoresis. A fungal reference map displaying 438 spots was set up. Four proteins, among the 14 selected for tandem mass spectrometry analysis, were identified including a NmrA‐like protein, an oxido‐reductase, a heat‐shock protein and an ATP synthase β mitochondrial precursor. The possible fungal origin of a MYK15‐like protein found in mycorrhizal roots was further discussed. This is the first report of arbuscular mycorrhizal fungal protein identifications by using a proteomic approach.