Proteomics as a way to identify extra‐radicular fungal proteins from Glomus intraradices – RiT‐DNA carrot root mycorrhizas

To identify fungal proteins involved in the arbuscular mycorrhizal symbiosis, root‐inducing transferred‐DNA transformed roots of carrot ( Daucus carota L.) were in vitro inoculated with Glomus intraradices . Proteins extracted from the extra‐radical fungus were analysed by two‐dimensional gel electrophoresis. A fungal reference map displaying 438 spots was set up. Four proteins, among the 14 selected for tandem mass spectrometry analysis, were identified including a NmrA‐like protein, an oxido‐reductase, a heat‐shock protein and an ATP synthase β mitochondrial precursor. The possible fungal origin of a MYK15‐like protein found in mycorrhizal roots was further discussed. This is the first report of arbuscular mycorrhizal fungal protein identifications by using a proteomic approach.