Cloning, structural analysis and expression of fast twitch skeletal muscle troponinC2 (TNNC2) gene in goat

TroponinC2 (TNNC2), a constituent of the troponin complex located on the thin filament, plays a critical role in skeletal muscle contraction and improving meat quality traits of livestock and poultry. The TNNC2 gene has been identified in many mammals, but very little known about goat. In the study, we cloned TNNC2 gene in goat for the first time and analyzed its tissue expression. Results indicated that TNNC2 contains 483bp CDS and encodes 160 amino acids, and it was selectively expressed in the muscular tissues of goat. Its nucleotide sequence was high similar with sheep (93.38%) and alpaca (90.48%), and the identity of encoded amino acids which was varied from 100% (sheep) to 70.81% (Atlantic salmon) in animal. We found that the Ala and leu was distributed very law in mammals. The amino acid residue of Ca binding sitesIIwas identical in mammals, Aves and amphibian. This study may provide more insight into the molecular structure and expression patterns of the vertebrate TNNC2 gene. Keywords-TroponinC2 gene; Molecular cloning; Structural analysis; Expression; Goat