Pelvic Chlamydial Infection Predisposes to Ectopic Pregnancy by Upregulating Integrin β1 to Promote Embryo-tubal Attachment | Zendy

Syed F. Ahmad | Zendy; Jeremy K. Brown | Zendy; Lisa Campbell | Zendy; Magda Koscielniak | Zendy; Catriona Oliver | Zendy; Nick Wheelhouse | Zendy; Gary Entrican | Zendy; Stuart McFee | Zendy; Gillian Wills | Zendy; Myra O. McClure | Zendy; Paddy Horner | Zendy; Sevasti Gaikoumelou | Zendy; Ckf Lee | Zendy; Hilary Critchley | Zendy; W. Colin Duncan | Zendy; Andrew W. Horne | Zendy

Open Access

Pelvic Chlamydial Infection Predisposes to Ectopic Pregnancy by Upregulating Integrin β1 to Promote Embryo-tubal Attachment

Author(s) -

Syed F. Ahmad,

Jeremy K. Brown,

Lisa Campbell,

Magda Koscielniak,

Catriona Oliver,

Nick Wheelhouse,

Gary Entrican,

Stuart McFee,

Gillian Wills,

Myra O. McClure,

Paddy Horner,

Sevasti Gaikoumelou,

Ckf Lee,

Hilary Critchley,

W. Colin Duncan,

Andrew W. Horne

Publication year - 2018

Publication title -

ebiomedicine

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.596

H-Index - 63

ISSN - 2352-3964

DOI - 10.1016/j.ebiom.2018.02.020

Subject(s) - chlamydia trachomatis , trophoblast , oviduct , embryo , fallopian tube , ectopic pregnancy , biology , integrin , andrology , uterus , embryo transfer , pregnancy , immunology , medicine , cell , microbiology and biotechnology , endocrinology , fetus , anatomy , genetics , placenta

Tubal ectopic pregnancies are a leading cause of global maternal morbidity and mortality. Previous infection with Chlamydia trachomatis is a major risk factor for tubal embryo implantation but the biological mechanism behind this association is unclear. Successful intra-uterine embryo implantation is associated with increased expression of endometrial "receptivity" integrins (cell adhesion molecules). We examined integrin expression in Fallopian tubes of women with previous C. trachomatis infection, in mice experimentally infected with C. trachomatis, in immortalised human oviductal epithelial cells (OE-E6/E7) and in an in vitro model of human embryo attachment (trophoblast spheroid-OE-E6/7 cell co-culture). Previous exposure with C. trachomatis increased Fallopian tube/oviduct integrin-subunit beta-1 (ITGB1) in women and mice compared to controls. C. trachomatis increased OE-E6/E7 cell ITGB1 expression and promoted trophoblast attachment to OE-E6/E7 cells which was negated by anti-ITGB1-antibody. We demonstrate that infection with C. trachomatis increases tubal ITGB1 expression, predisposing to tubal embryo attachment and ectopic pregnancy.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research