Vinculin expression in MC3T3-E1 cells in response to mechanical stimulus | Zendy

Jose Cora-Cruz | Zendy; N. DiffootCarlo | Zendy; P.A. Sundaram | Zendy

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Vinculin expression in MC3T3-E1 cells in response to mechanical stimulus

Author(s) -

Jose Cora-Cruz,

N. DiffootCarlo,

P.A. Sundaram

Publication year - 2015

Publication title -

data in brief

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.122

H-Index - 30

ISSN - 2352-3409

DOI - 10.1016/j.dib.2015.11.052

Subject(s) - vinculin , focal adhesion , cytoplasm , mechanotransduction , microbiology and biotechnology , immunofluorescence , cell mechanics , chemistry , stimulation , adhesion , osteoblast , cell adhesion , indirect immunofluorescence , biophysics , cytoskeleton , cell , biology , signal transduction , in vitro , antibody , biochemistry , immunology , neuroscience , organic chemistry

Loading frequency is known to influence the expression of the focal adhesions of the adherent cells. A small cyclical tensile force was transmitted to mouse pre-osteoblast MC3T3-E1 cells through PDMS substrates of varying stiffness. Changes in cell behavior with respect to proliferation and characteristics of focal adhesions were quantified through immunofluorescence labeling of vinculin. Amount of inactive vinculin was higher on substrates subjected to cyclic stimulation when compared with the results of the static substrates, whereas the number and area of focal adhesion points underwent a reduction. Inactive vinculin appears as a cloud in the cytoplasm in the vicinity of the nucleus.

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