Data from proteomic characterization of the role of Snail1 in murine mesenchymal stem cells and 3T3-L1 fibroblasts differentiation | Zendy

Alberto PeláezGarcía | Zendy; Rodrigo Barderas | Zendy; Marta Mendes | Zendy; María López-Lucendo | Zendy; Juana Sánchez | Zendy; Antonio Garcı́a de Herreros | Zendy; J. Ignacio Casal | Zendy

Open Access

Data from proteomic characterization of the role of Snail1 in murine mesenchymal stem cells and 3T3-L1 fibroblasts differentiation

Author(s) -

Alberto PeláezGarcía,

Rodrigo Barderas,

Marta Mendes,

María López-Lucendo,

Juana Sánchez,

Antonio Garcı́a de Herreros,

J. Ignacio Casal

Publication year - 2015

Publication title -

data in brief

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.122

H-Index - 30

ISSN - 2352-3409

DOI - 10.1016/j.dib.2015.07.027

Subject(s) - mesenchymal stem cell , biology , microbiology and biotechnology , proteomics , embryonic stem cell , cellular differentiation , stable isotope labeling by amino acids in cell culture , genetics , gene

The transcription factor (TF) Snail1 is a major inducer of the epithelial-mesenchymal transition (EMT) during embryonic development and cancer progression. Ectopic expression of Snail in murine mesenchymal stem cells (mMSC) abrogated their differentiation to osteoblasts or adipocytes. We used either stable isotopic metabolic labeling (SILAC) for 3T3-L1 cells or isobaric labeling with tandem mass tags (TMT) for mMSC stably transfected cells with Snail1 or control. We carried out a proteomic analysis on the nuclear fraction since Snail is a nuclear TF that mediates its effects mainly through the regulation of other TFs. Proteomics data have been deposited in ProteomeXchange via the PRIDE partner repository with the dataset identifiers PXD001529 and PXD002157 (Vizcaino et al., 2014) [1]. Data are associated with a research article published in Molecular and Cellular Proteomics (Pelaez-Garcia et al., 2015) [2].

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