A MITE insertion into the 3′-UTR regulates the transcription of TaHSP16.9 in common wheat | Zendy

Jingting Li | Zendy; Zhenzhong Wang | Zendy; Huiru Peng | Zendy; Zhiyong Liu | Zendy

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A MITE insertion into the 3′-UTR regulates the transcription of TaHSP16.9 in common wheat

Author(s) -

Jingting Li,

Zhenzhong Wang,

Huiru Peng,

Zhiyong Liu

Publication year - 2014

Publication title -

the crop journal

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.437

H-Index - 29

eISSN - 2095-5421

pISSN - 2214-5141

DOI - 10.1016/j.cj.2014.07.001

Subject(s) - transposable element , biology , genetics , promoter , gene , untranslated region , intron , mutant , coding region , transcription (linguistics) , mite , microbiology and biotechnology , messenger rna , gene expression , linguistics , philosophy , botany

Miniature inverted-repeat transposable elements (MITEs) are a type of DNA transposon frequently inserted into promoters, untranslated regions (UTR), introns, or coding sequences of genes. We found a 276-bp tourist-like MITE insertion in the 3′-UTR of a 16.9kDa small heat shock protein gene (TaHSP16.9-3A) on chromosome 3A of common wheat. Haplotype analysis revealed two haplotypes, sHSP-W (wild type without MITE insertion) and sHSP-M (mutant with MITE insertion), present in wheat germplasm. Both semiquantitative PCR and quantitative real-time PCR analyses showed increased transcription levels of TaHSP16.9-3A in sHSP-M compared with those of sHSP-W after heat treatment at 42°C. It appeared that the MITE insertion into the 3′-UTR enhances the transcription of TaHSP16.9-3A

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