Microcircuit mechanisms for the generation of sharp-wave ripples in the basolateral amygdala: A role for chandelier interneurons

Synchronized activity in neural circuits, detected as oscillations in the extracellular field potential, has been associated with learning and memory. Neural circuits in the basolateral amygdala (BLA), a mid-temporal lobe structure, generate oscillations in specific frequency bands to mediate emotional memory functions. However, how BLA circuits generate oscillations in distinct frequency bands is not known. Of these, sharp-waves (SWs) are repetitive, brief transitions that contain a low-frequency (<20 Hz) envelope, often coupled with ripples (100-300 Hz), have been associated with memory consolidation. Here, we show that SWs are retained in the BLA ex vivo and generated by local circuits. We demonstrate that an action potential in a chandelier interneuron is sufficient to initiate SWs through local circuits. Using a physiologically constrained model, we show that microcircuits organized as chandelier-interneuron-driven modules reproduce SWs and associated cellular events, revealing a functional role for chandelier interneurons and microcircuits for SW generation.