Open AccessA Chemical Screen Identifies Compounds Capable of Selecting for Haploidy in Mammalian Cells
Author(s) -
Teresa Olbrich,
María Vega-Sendino,
Matilde Murga,
Guillermo de Cárcer,
Marcos Malumbres,
Sagrario Ortega,
Sergio Ruiz,
Óscar Fernández-Capetillo
Publication year - 2019
Publication title -
cell reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.264
H-Index - 154
eISSN - 2639-1856
pISSN - 2211-1247
DOI - 10.1016/j.celrep.2019.06.060
Subject(s) - ploidy , somatic cell , biology , cell culture , embryonic stem cell , phenotype , microbiology and biotechnology , cell , genetics , gene
The recent availability of somatic haploid cell lines has provided a unique tool for genetic studies in mammals. However, the percentage of haploid cells rapidly decreases in these cell lines, which we recently showed is due to their overgrowth by diploid cells present in the cultures. Based on this property, we have now performed a phenotypic chemical screen in human haploid HAP1 cells aiming to identify compounds that facilitate the maintenance of haploid cells. Our top hit was 10-Deacetyl-baccatin-III (DAB), a chemical precursor in the synthesis of Taxol, which selects for haploid cells in HAP1 and mouse haploid embryonic stem cultures. Interestingly, DAB also enriches for diploid cells in mixed cultures of diploid and tetraploid cells, including in the colon cancer cell line DLD-1, revealing a general strategy for selecting cells with lower ploidy in mixed populations of mammalian cells.
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