Molecular Profiling and Functional Analysis of Macrophage-Derived Tumor Extracellular Vesicles | Zendy

Chiara Cianciaruso | Zendy; Tim Beltraminelli | Zendy; Florent Duval | Zendy; Sissiri | Zendy; Romain Hamelin | Zendy; André Mozes | Zendy; Héctor GallartAyala | Zendy; Gerardo Ceada | Zendy; Bruno Torchia | Zendy; Carola H. Ries | Zendy; Julijana Ivanišević | Zendy; Michele De Palma | Zendy

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Molecular Profiling and Functional Analysis of Macrophage-Derived Tumor Extracellular Vesicles

Author(s) -

Chiara Cianciaruso,

Tim Beltraminelli,

Florent Duval,

Sissiri,

Romain Hamelin,

André Mozes,

Héctor GallartAyala,

Gerardo Ceada,

Bruno Torchia,

Carola H. Ries,

Julijana Ivanišević,

Michele De Palma

Publication year - 2019

Publication title -

cell reports

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 6.264

H-Index - 154

eISSN - 2639-1856

pISSN - 2211-1247

DOI - 10.1016/j.celrep.2019.05.008

Subject(s) - microvesicles , immune system , tumor microenvironment , inflammation , extracellular vesicle , extracellular vesicles , biology , secretion , exosome , macrophage polarization , microbiology and biotechnology , cancer research , ex vivo , macrophage , chemistry , microrna , immunology , in vitro , biochemistry , gene

Extracellular vesicles (EVs), including exosomes, modulate multiple aspects of cancer biology. Tumor-associated macrophages (TAMs) secrete EVs, but their molecular features and functions are poorly characterized. Here, we report methodology for the enrichment, quantification, and proteomic and lipidomic analysis of EVs released from mouse TAMs (TAM-EVs). Compared to source TAMs, TAM-EVs present molecular profiles associated with a Th1/M1 polarization signature, enhanced inflammation and immune response, and a more favorable patient prognosis. Accordingly, enriched TAM-EV preparations promote T cell proliferation and activation ex vivo. TAM-EVs also contain bioactive lipids and biosynthetic enzymes, which may alter pro-inflammatory signaling in the cancer cells. Thus, whereas TAMs are largely immunosuppressive, their EVs may have the potential to stimulate, rather than limit, anti-tumor immunity.

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