Nidogen-1 Contributes to the Interaction Network Involved in Pro-B Cell Retention in the Peri-sinusoidal Hematopoietic Stem Cell Niche | Zendy

Marielle Balzano | Zendy; Maria De Grandis | Zendy; ThienPhong Vu Manh | Zendy; Lionel Chasson | Zendy; Florence Bardin | Zendy; Anne Farina | Zendy; Arnauld Sergé | Zendy; Ghislain Bidaut | Zendy; Pierre Charbord | Zendy; Léonard Hérault | Zendy; Anne-Laure Bailly | Zendy; Amandine Cartier-Michaud | Zendy; Annie Boned | Zendy; Marc Dalod | Zendy; Estelle Duprez | Zendy; Paul G. Genever | Zendy; Mark Coles | Zendy; Marc Bajénoff | Zendy; Luc Xerri | Zendy; Michel AurrandLions | Zendy; Claudine Schiff | Zendy; Stéphane J.C. Mancini | Zendy

Open Access

Nidogen-1 Contributes to the Interaction Network Involved in Pro-B Cell Retention in the Peri-sinusoidal Hematopoietic Stem Cell Niche

Author(s) -

Marielle Balzano,

Maria De Grandis,

ThienPhong Vu Manh,

Lionel Chasson,

Florence Bardin,

Anne Farina,

Arnauld Sergé,

Ghislain Bidaut,

Pierre Charbord,

Léonard Hérault,

Anne-Laure Bailly,

Amandine Cartier-Michaud,

Annie Boned,

Marc Dalod,

Estelle Duprez,

Paul G. Genever,

Mark Coles,

Marc Bajénoff,

Luc Xerri,

Michel AurrandLions,

Claudine Schiff,

Stéphane J.C. Mancini

Publication year - 2019

Publication title -

cell reports

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 6.264

H-Index - 154

eISSN - 2639-1856

pISSN - 2211-1247

DOI - 10.1016/j.celrep.2019.02.065

Subject(s) - peri , niche , haematopoiesis , microbiology and biotechnology , hematopoietic stem cell , stem cell , cell , stem cell niche , biology , genetics , ecology , medicine , progenitor cell

In the bone marrow, CXCL12 and IL-7 are essential for B cell differentiation, whereas hematopoietic stem cell (HSC) maintenance requires SCF and CXCL12. Peri-sinusoidal stromal (PSS) cells are the main source of IL-7, but their characterization as a pro-B cell niche remains limited. Here, we characterize pro-B cell supporting stromal cells and decipher the interaction network allowing pro-B cell retention. Preferential contacts are found between pro-B cells and PSS cells, which homogeneously express HSC and B cell niche genes. Furthermore, pro-B cells are frequently located in the vicinity of HSCs in the same niche. Using an interactome bioinformatics pipeline, we identify Nidogen-1 as essential for pro-B cell retention in the peri-sinusoidal niche as confirmed in Nidogen-1 -/- mice. Finally, human pro-B cells and hematopoietic progenitors are observed close to similar IL-7 + stromal cells. Thus, a multispecific niche exists in mouse and human supporting both early progenitors and committed hematopoietic lineages.

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