Transient N-6-Methyladenosine Transcriptome Sequencing Reveals a Regulatory Role of m6A in Splicing Efficiency | Zendy

Annita Louloupi | Zendy; Evgenia Ntini | Zendy; Thomas Conrad | Zendy; Ulf Andersson Ørom | Zendy

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Transient N-6-Methyladenosine Transcriptome Sequencing Reveals a Regulatory Role of m6A in Splicing Efficiency

Author(s) -

Annita Louloupi,

Evgenia Ntini,

Thomas Conrad,

Ulf Andersson Ørom

Publication year - 2018

Publication title -

cell reports

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 6.264

H-Index - 154

eISSN - 2639-1856

pISSN - 2211-1247

DOI - 10.1016/j.celrep.2018.05.077

Subject(s) - rna splicing , intron , alternative splicing , rna , biology , exonic splicing enhancer , n6 methyladenosine , splicing factor , microbiology and biotechnology , exon , rna binding protein , gene , genetics , methylation , methyltransferase

Splicing efficiency varies among transcripts, and tight control of splicing kinetics is crucial for coordinated gene expression. N-6-methyladenosine (m6A) is the most abundant RNA modification and is involved in regulation of RNA biogenesis and function. The impact of m6A on regulation of RNA splicing kinetics is unknown. Here, we provide a time-resolved high-resolution assessment of m6A on nascent RNA transcripts and unveil its importance for the control of RNA splicing kinetics. We find that early co-transcriptional m6A deposition near splice junctions promotes fast splicing, while m6A modifications in introns are associated with long, slowly processed introns and alternative splicing events. In conclusion, we show that early m6A deposition specifies the fate of transcripts regarding splicing kinetics and alternative splicing.

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