Potency Analysis of Mesenchymal Stromal Cells Using a Combinatorial Assay Matrix Approach | Zendy

Raghavan Chinnadurai | Zendy; Devi Rajan | Zendy; Muna Qayed | Zendy; Dalia Arafat | Zendy; Marco Garcia | Zendy; Yifei Liu | Zendy; Subra Kugathasan | Zendy; Larry J. Anderson | Zendy; Greg Gibson | Zendy; Jacques Galipeau | Zendy

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Potency Analysis of Mesenchymal Stromal Cells Using a Combinatorial Assay Matrix Approach

Author(s) -

Raghavan Chinnadurai,

Devi Rajan,

Muna Qayed,

Dalia Arafat,

Marco Garcia,

Yifei Liu,

Subra Kugathasan,

Larry J. Anderson,

Greg Gibson,

Jacques Galipeau

Publication year - 2018

Publication title -

cell reports

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 6.264

H-Index - 154

eISSN - 2639-1856

pISSN - 2211-1247

DOI - 10.1016/j.celrep.2018.02.013

Subject(s) - potency , mesenchymal stem cell , immune system , peripheral blood mononuclear cell , cytokine , homing (biology) , biology , immunology , computational biology , microbiology and biotechnology , in vitro , biochemistry , ecology

Assays that can characterize MSC immune potency need to be identified for use in advanced clinical trials. MSCs possess a number of putative regenerative and immunomodulatory properties, and an assay matrix approach may best capture involved effector pathways. We have tested two assay systems to measure the potency of MSCs derived from human subjects: MSC secretome analysis and a quantitative RNA-based array for genes specific to immunomodulatory and homing properties of MSCs. Secretome analysis identified a unique cytokine signature that is upregulated by MSCs or downregulated in responder PBMCs and correlated with T cell suppression. Use of interferon-γ as a surrogate for the action of activated PBMCs on MSCs served as an alternative for the use of human PBMCs as responder cells in a potency assay. Our approach and results define and simplify the multifunctional or matrix responses of MSCs and may serve as a platform for robust potency analysis.

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