Systematic Profiling of Histone Readers in Arabidopsis thaliana | Zendy

Shuai Zhao | Zendy; Baichao Zhang | Zendy; Mo Yang | Zendy; Jinsong Zhu | Zendy; Haitao Li | Zendy

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Systematic Profiling of Histone Readers in Arabidopsis thaliana

Author(s) -

Shuai Zhao,

Baichao Zhang,

Mo Yang,

Jinsong Zhu,

Haitao Li

Publication year - 2018

Publication title -

cell reports

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 6.264

H-Index - 154

eISSN - 2639-1856

pISSN - 2211-1247

DOI - 10.1016/j.celrep.2017.12.099

Subject(s) - histone , biology , histone methylation , dna damage repair , histone h2a , computational biology , histone code , epigenomics , arabidopsis , histone methyltransferase , arabidopsis thaliana , genetics , histone h1 , microbiology and biotechnology , dna , dna repair , dna methylation , gene , gene expression , nucleosome , mutant

Histone post-translational modifications (PTMs) and their recognition by histone readers exert crucial functions in eukaryotes. Despite extensive studies, conservation and diversity of histone PTM regulation between animals and plants remain less explored because of a lack of systematic knowledge of histone readers in plants. Based on a high-throughput surface plasmon resonance imaging (SPRi) platform, we report the lab-on-chip profiling of interactions between 204 putative reader domains and 11 types of histone peptides in Arabidopsis thaliana. Eleven reader hits were then chosen for histone combinatorial readout pattern profiling. Systematic analysis of histone PTM recognition in Arabidopsis thaliana reveals that plant and human histone readers share conservation in domain types and recognition mechanisms. The differences in particular histone mark recognition by transcription regulator EML1 and DNA damage repair factor MSH6 indicate plant-specific histone PTMs function in Arabidopsis thaliana acquired during evolution.

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