Citrobacter rodentium Relies on Commensals for Colonization of the Colonic Mucosa | Zendy

Caroline Mullineaux-Sanders | Zendy; James W. Collins | Zendy; David RuanoGallego | Zendy; Maayan Levy | Zendy; Meirav PevsnerFischer | Zendy; Izabela Glegola-Madejska | Zendy; Agnes Sagfors | Zendy; Joshua L. C. Wong | Zendy; Eran Elinav | Zendy; Valérie F. Crepin | Zendy; Gad Frankel | Zendy

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Citrobacter rodentium Relies on Commensals for Colonization of the Colonic Mucosa

Author(s) -

Caroline Mullineaux-Sanders,

James W. Collins,

David RuanoGallego,

Maayan Levy,

Meirav PevsnerFischer,

Izabela Glegola-Madejska,

Agnes Sagfors,

Joshua L. C. Wong,

Eran Elinav,

Valérie F. Crepin,

Gad Frankel

Publication year - 2017

Publication title -

cell reports

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 6.264

H-Index - 154

eISSN - 2639-1856

pISSN - 2211-1247

DOI - 10.1016/j.celrep.2017.11.086

Subject(s) - citrobacter rodentium , commensalism , colonization , citrobacter , biology , intestinal mucosa , microbiology and biotechnology , medicine , bacteria , enterobacteriaceae , pathogen , escherichia coli , genetics , gene , biochemistry

We investigated the role of commensals at the peak of infection with the colonic mouse pathogen Citrobacter rodentium. Bioluminescent and kanamycin (Kan)-resistant C. rodentium persisted avirulently in the cecal lumen of mice continuously treated with Kan. A single Kan treatment was sufficient to displace C. rodentium from the colonic mucosa, a phenomenon not observed following treatment with vancomycin (Van) or metronidazole (Met). Kan, Van, and Met induce distinct dysbiosis, suggesting C. rodentium relies on specific commensals for colonic colonization. Expression of the master virulence regulator ler is induced in germ-free mice, yet C. rodentium is only seen in the cecal lumen. Moreover, in conventional mice, a single Kan treatment was sufficient to displace C. rodentium constitutively expressing Ler from the colonic mucosa. These results show that expression of virulence genes is not sufficient for colonization of the colonic mucosa and that commensals are essential for a physiological infection course.

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