Dual RNA Processing Roles of Pat1b via Cytoplasmic Lsm1-7 and Nuclear Lsm2-8 Complexes | Zendy

Caroline Vindry | Zendy; Aline Marnef | Zendy; Helen Broomhead | Zendy; Laure Twyffels | Zendy; Sevim Ozgur | Zendy; Georg Stoecklin | Zendy; Miriam Llorian | Zendy; Christopher W. J. Smith | Zendy; Juan Mata | Zendy; Dominique Weil | Zendy; Nancy Standart | Zendy

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Dual RNA Processing Roles of Pat1b via Cytoplasmic Lsm1-7 and Nuclear Lsm2-8 Complexes

Author(s) -

Caroline Vindry,

Aline Marnef,

Helen Broomhead,

Laure Twyffels,

Sevim Ozgur,

Georg Stoecklin,

Miriam Llorian,

Christopher W. J. Smith,

Juan Mata,

Dominique Weil,

Nancy Standart

Publication year - 2017

Publication title -

cell reports

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 6.264

H-Index - 154

eISSN - 2639-1856

pISSN - 2211-1247

DOI - 10.1016/j.celrep.2017.06.091

Subject(s) - snrnp , small nuclear rna , rna , rna splicing , microbiology and biotechnology , cajal body , small nuclear ribonucleoprotein , biology , ribonucleoprotein , rna binding protein , precursor mrna , intron , spliceosome , messenger rna , heterogeneous ribonucleoprotein particle , non coding rna , genetics , gene

Pat1 RNA-binding proteins, enriched in processing bodies (P bodies), are key players in cytoplasmic 5' to 3' mRNA decay, activating decapping of mRNA in complex with the Lsm1-7 heptamer. Using co-immunoprecipitation and immunofluorescence approaches coupled with RNAi, we provide evidence for a nuclear complex of Pat1b with the Lsm2-8 heptamer, which binds to the spliceosomal U6 small nuclear RNA (snRNA). Furthermore, we establish the set of interactions connecting Pat1b/Lsm2-8/U6 snRNA/SART3 and additional U4/U6.U5 tri-small nuclear ribonucleoprotein particle (tri-snRNP) components in Cajal bodies, the site of snRNP biogenesis. RNA sequencing following Pat1b depletion revealed the preferential upregulation of mRNAs normally found in P bodies and enriched in 3' UTR AU-rich elements. Changes in >180 alternative splicing events were also observed, characterized by skipping of regulated exons with weak donor sites. Our data demonstrate the dual role of a decapping enhancer in pre-mRNA processing as well as in mRNA decay via distinct nuclear and cytoplasmic Lsm complexes.

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