DOC1-Dependent Recruitment of NURD Reveals Antagonism with SWI/SNF during Epithelial-Mesenchymal Transition in Oral Cancer Cells | Zendy

Adone Mohd-Sarip | Zendy; Miriam Teeuwssen | Zendy; Alice G.M. Bot | Zendy; Maria J. De Herdt | Zendy; Stefan M. Willems | Zendy; Robert J. Baatenburg de Jong | Zendy; Leendert H. J. Looijenga | Zendy; Diana Zatreanu | Zendy; Karel Bezstarosti | Zendy; Job van Riet | Zendy; Edwin Oole | Zendy; Wilfred F. J. van IJcken | Zendy; Harmen J.G. van de Werken | Zendy; Jeroen Demmers | Zendy; Riccardo Fodde | Zendy; C. Peter Verrijzer | Zendy

Open Access

DOC1-Dependent Recruitment of NURD Reveals Antagonism with SWI/SNF during Epithelial-Mesenchymal Transition in Oral Cancer Cells

Author(s) -

Adone Mohd-Sarip,

Miriam Teeuwssen,

Alice G.M. Bot,

Maria J. De Herdt,

Stefan M. Willems,

Robert J. Baatenburg de Jong,

Leendert H. J. Looijenga,

Diana Zatreanu,

Karel Bezstarosti,

Job van Riet,

Edwin Oole,

Wilfred F. J. van IJcken,

Harmen J.G. van de Werken,

Jeroen Demmers,

Riccardo Fodde,

C. Peter Verrijzer

Publication year - 2017

Publication title -

cell reports

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 6.264

H-Index - 154

eISSN - 2639-1856

pISSN - 2211-1247

DOI - 10.1016/j.celrep.2017.06.020

Subject(s) - swi/snf , chromatin , microbiology and biotechnology , prc2 , reprogramming , epithelial–mesenchymal transition , biology , chromatin remodeling , epigenetics , histone , transcription factor , carcinogenesis , nucleosome , cancer research , histone h3 , genetics , cell , transition (genetics) , cancer , gene

The Nucleosome Remodeling and Deacetylase (NURD) complex is a key regulator of cell differentiation that has also been implicated in tumorigenesis. Loss of the NURD subunit Deleted in Oral Cancer 1 (DOC1) is associated with human oral squamous cell carcinomas (OSCCs). Here, we show that restoration of DOC1 expression in OSCC cells leads to a reversal of epithelial-mesenchymal transition (EMT). This is caused by the DOC1-dependent targeting of NURD to repress key transcriptional regulators of EMT. NURD recruitment drives extensive epigenetic reprogramming, including eviction of the SWI/SNF remodeler, formation of inaccessible chromatin, H3K27 deacetylation, and binding of PRC2 and KDM1A, followed by H3K27 methylation and H3K4 demethylation. Strikingly, depletion of SWI/SNF mimics the effects of DOC1 re-expression. Our results suggest that SWI/SNF and NURD function antagonistically to control chromatin state and transcription. We propose that disturbance of this dynamic equilibrium may lead to defects in gene expression that promote oncogenesis.

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