Specification and Diversification of Pericytes and Smooth Muscle Cells from Mesenchymoangioblasts | Zendy

Akhilesh Kumar | Zendy; Saritha S. D’Souza | Zendy; Oleg V. Moskvin | Zendy; Huishi Toh | Zendy; Bowen Wang | Zendy; Jue Zhang | Zendy; Scott Swanson | Zendy; Lian-Wang Guo | Zendy; James A. Thomson | Zendy; Igor I. Slukvin | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Specification and Diversification of Pericytes and Smooth Muscle Cells from Mesenchymoangioblasts

Author(s) -

Akhilesh Kumar,

Saritha S. D’Souza,

Oleg V. Moskvin,

Huishi Toh,

Bowen Wang,

Jue Zhang,

Scott Swanson,

Lian-Wang Guo,

James A. Thomson,

Igor I. Slukvin

Publication year - 2017

Publication title -

cell reports

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 6.264

H-Index - 154

eISSN - 2639-1856

pISSN - 2211-1247

DOI - 10.1016/j.celrep.2017.05.019

Subject(s) - mesenchymal stem cell , biology , microbiology and biotechnology , progenitor cell , mural cell , pericyte , induced pluripotent stem cell , stem cell , mesoderm , stromal cell , vascular smooth muscle , endothelial stem cell , immunology , cancer research , embryonic stem cell , genetics , smooth muscle , endocrinology , in vitro , gene

Elucidating the pathways that lead to vasculogenic cells, and being able to identify their progenitors and lineage-restricted cells, is critical to the establishment of human pluripotent stem cell (hPSC) models for vascular diseases and development of vascular therapies. Here, we find that mesoderm-derived pericytes (PCs) and smooth muscle cells (SMCs) originate from a clonal mesenchymal progenitor mesenchymoangioblast (MB). In clonogenic cultures, MBs differentiate into primitive PDGFRβ + CD271 + CD73 - mesenchymal progenitors, which give rise to proliferative PCs, SMCs, and mesenchymal stem/stromal cells. MB-derived PCs can be further specified to CD274 + capillary and DLK1 + arteriolar PCs with a proinflammatory and contractile phenotype, respectively. SMC maturation was induced using a MEK inhibitor. Establishing the vasculogenic lineage tree, along with identification of stage- and lineage-specific markers, provides a platform for interrogating the molecular mechanisms that regulate vasculogenic cell specification and diversification and manufacturing well-defined mural cell populations for vascular engineering and cellular therapies from hPSCs.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research