A Temporal Proteomic Map of Epstein-Barr Virus Lytic Replication in B Cells | Zendy

Ina Ersing | Zendy; Luís Nobre | Zendy; Liang Wei Wang | Zendy; Lior Soday | Zendy; Yijie Ma | Zendy; João A. Paulo | Zendy; Yohei Narita | Zendy; Camille W. Ashbaugh | Zendy; Chang Jiang | Zendy; Nicholas E. Grayson | Zendy; Elliott Kieff | Zendy; Steven P. Gygi | Zendy; Michael P. Weekes | Zendy; Benjamin E. Gewurz | Zendy

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A Temporal Proteomic Map of Epstein-Barr Virus Lytic Replication in B Cells

Author(s) -

Ina Ersing,

Luís Nobre,

Liang Wei Wang,

Lior Soday,

Yijie Ma,

João A. Paulo,

Yohei Narita,

Camille W. Ashbaugh,

Chang Jiang,

Nicholas E. Grayson,

Elliott Kieff,

Steven P. Gygi,

Michael P. Weekes,

Benjamin E. Gewurz

Publication year - 2017

Publication title -

cell reports

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 6.264

H-Index - 154

eISSN - 2639-1856

pISSN - 2211-1247

DOI - 10.1016/j.celrep.2017.04.062

Subject(s) - lytic cycle , biology , epstein–barr virus , virus , virology , mononucleosis , nasopharyngeal carcinoma , proteome , lymphoma , viral replication , immunology , genetics , medicine , radiation therapy

Epstein-Barr virus (EBV) replication contributes to multiple human diseases, including infectious mononucleosis, nasopharyngeal carcinoma, B cell lymphomas, and oral hairy leukoplakia. We performed systematic quantitative analyses of temporal changes in host and EBV proteins during lytic replication to gain insights into virus-host interactions, using conditional Burkitt lymphoma models of type I and II EBV infection. We quantified profiles of >8,000 cellular and 69 EBV proteins, including >500 plasma membrane proteins, providing temporal views of the lytic B cell proteome and EBV virome. Our approach revealed EBV-induced remodeling of cell cycle, innate and adaptive immune pathways, including upregulation of the complement cascade and proteasomal degradation of the B cell receptor complex, conserved between EBV types I and II. Cross-comparison with proteomic analyses of human cytomegalovirus infection and of a Kaposi-sarcoma-associated herpesvirus immunoevasin identified host factors targeted by multiple herpesviruses. Our results provide an important resource for studies of EBV replication.

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