Primary Cell Culture of Live Neurosurgically Resected Aged Adult Human Brain Cells and Single Cell Transcriptomics | Zendy

Jennifer Spaethling | Zendy; Young-Ji Na | Zendy; Jaehee Lee | Zendy; Alexandra V. Ulyanova | Zendy; Gordon H. Baltuch | Zendy; Thomas J. Bell | Zendy; Steven Brem | Zendy; H. Isaac Chen | Zendy; Hannah Dueck | Zendy; Stephen Fisher | Zendy; Marcela P. Garcia | Zendy; Mugdha Khaladkar | Zendy; David Kung | Zendy; Timothy H. Lucas | Zendy; Donald M. O’Rourke | Zendy; Derek Stefanik | Zendy; Jinhui Wang | Zendy; John A. Wolf | Zendy; Tamás Bartfai | Zendy; M. Sean Grady | Zendy; JaiYoon Sul | Zendy; Junhyong Kim | Zendy; James Eberwine | Zendy

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Primary Cell Culture of Live Neurosurgically Resected Aged Adult Human Brain Cells and Single Cell Transcriptomics

Author(s) -

Jennifer Spaethling,

Young-Ji Na,

Jaehee Lee,

Alexandra V. Ulyanova,

Gordon H. Baltuch,

Thomas J. Bell,

Steven Brem,

H. Isaac Chen,

Hannah Dueck,

Stephen Fisher,

Marcela P. Garcia,

Mugdha Khaladkar,

David Kung,

Timothy H. Lucas,

Donald M. O’Rourke,

Derek Stefanik,

Jinhui Wang,

John A. Wolf,

Tamás Bartfai,

M. Sean Grady,

JaiYoon Sul,

Junhyong Kim,

James Eberwine

Publication year - 2017

Publication title -

cell reports

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 6.264

H-Index - 154

eISSN - 2639-1856

pISSN - 2211-1247

DOI - 10.1016/j.celrep.2016.12.066

Subject(s) - transcriptome , cell type , biology , cell , microglia , human brain , cell culture , microbiology and biotechnology , computational biology , gene , gene expression , neuroscience , immunology , genetics , inflammation

Investigation of human CNS disease and drug effects has been hampered by the lack of a system that enables single-cell analysis of live adult patient brain cells. We developed a culturing system, based on a papain-aided procedure, for resected adult human brain tissue removed during neurosurgery. We performed single-cell transcriptomics on over 300 cells, permitting identification of oligodendrocytes, microglia, neurons, endothelial cells, and astrocytes after 3 weeks in culture. Using deep sequencing, we detected over 12,000 expressed genes, including hundreds of cell-type-enriched mRNAs, lncRNAs and pri-miRNAs. We describe cell-type- and patient-specific transcriptional hierarchies. Single-cell transcriptomics on cultured live adult patient derived cells is a prime example of the promise of personalized precision medicine. Because these cells derive from subjects ranging in age into their sixties, this system permits human aging studies previously possible only in rodent systems.

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