Neonatal Transplantation Confers Maturation of PSC-Derived Cardiomyocytes Conducive to Modeling Cardiomyopathy | Zendy

Gun-Sik Cho | Zendy; Dong I. Lee | Zendy; Emmanouil Tampakakis | Zendy; Sean Murphy | Zendy; Peter Andersen | Zendy; Hideki Uosaki | Zendy; Stephen P. Chelko | Zendy; Khalid Chakir | Zendy; Ingie Hong | Zendy; Kinya Seo | Zendy; Huei-Sheng Vincent Chen | Zendy; Xiongwen Chen | Zendy; Cristina Basso | Zendy; Steven R. Houser | Zendy; Gordon F. Tomaselli | Zendy; Brian O’Rourke | Zendy; Daniel P. Judge | Zendy; David A. Kass | Zendy; Chulan Kwon | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Neonatal Transplantation Confers Maturation of PSC-Derived Cardiomyocytes Conducive to Modeling Cardiomyopathy

Author(s) -

Gun-Sik Cho,

Dong I. Lee,

Emmanouil Tampakakis,

Sean Murphy,

Peter Andersen,

Hideki Uosaki,

Stephen P. Chelko,

Khalid Chakir,

Ingie Hong,

Kinya Seo,

Huei-Sheng Vincent Chen,

Xiongwen Chen,

Cristina Basso,

Steven R. Houser,

Gordon F. Tomaselli,

Brian O’Rourke,

Daniel P. Judge,

David A. Kass,

Chulan Kwon

Publication year - 2017

Publication title -

cell reports

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 6.264

H-Index - 154

eISSN - 2639-1856

pISSN - 2211-1247

DOI - 10.1016/j.celrep.2016.12.040

Subject(s) - transplantation , induced pluripotent stem cell , cardiomyopathy , phenotype , pathogenesis , zebrafish , biology , embryonic stem cell , heart transplantation , disease , in vivo , heart disease , fetus , medicine , bioinformatics , microbiology and biotechnology , pathology , immunology , heart failure , genetics , gene , pregnancy

Pluripotent stem cells (PSCs) offer unprecedented opportunities for disease modeling and personalized medicine. However, PSC-derived cells exhibit fetal-like characteristics and remain immature in a dish. This has emerged as a major obstacle for their application for late-onset diseases. We previously showed that there is a neonatal arrest of long-term cultured PSC-derived cardiomyocytes (PSC-CMs). Here, we demonstrate that PSC-CMs mature into adult CMs when transplanted into neonatal hearts. PSC-CMs became similar to adult CMs in morphology, structure, and function within a month of transplantation into rats. The similarity was further supported by single-cell RNA-sequencing analysis. Moreover, this in vivo maturation allowed patient-derived PSC-CMs to reveal the disease phenotype of arrhythmogenic right ventricular cardiomyopathy, which manifests predominantly in adults. This study lays a foundation for understanding human CM maturation and pathogenesis and can be instrumental in PSC-based modeling of adult heart diseases.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research