MouSensor: A Versatile Genetic Platform to Create Super Sniffer Mice for Studying Human Odor Coding | Zendy

Charlotte D’Hulst | Zendy; Raena Mina | Zendy; Zachary Gershon | Zendy; Sophie Jamet | Zendy; Antonio R. Cerullo | Zendy; Delia Tomoiaga | Zendy; Li Bai | Zendy; Leonardo Belluscio | Zendy; Matthew E. Rogers | Zendy; Yevgeniy B. Sirotin | Zendy; Paul Feinstein | Zendy

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MouSensor: A Versatile Genetic Platform to Create Super Sniffer Mice for Studying Human Odor Coding

Author(s) -

Charlotte D’Hulst,

Raena Mina,

Zachary Gershon,

Sophie Jamet,

Antonio R. Cerullo,

Delia Tomoiaga,

Li Bai,

Leonardo Belluscio,

Matthew E. Rogers,

Yevgeniy B. Sirotin,

Paul Feinstein

Publication year - 2016

Publication title -

cell reports

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 6.264

H-Index - 154

eISSN - 2639-1856

pISSN - 2211-1247

DOI - 10.1016/j.celrep.2016.06.047

Subject(s) - olfactory bulb , biology , olfactory epithelium , enhancer , olfaction , olfactory system , neuroscience , sensory system , axon guidance , gene , odor , coding region , homeobox , receptor , transgene , in vivo , olfactory receptor , microbiology and biotechnology , axon , gene expression , genetics , central nervous system

Typically, ∼0.1% of the total number of olfactory sensory neurons (OSNs) in the main olfactory epithelium express the same odorant receptor (OR) in a singular fashion and their axons coalesce into homotypic glomeruli in the olfactory bulb. Here, we have dramatically increased the total number of OSNs expressing specific cloned OR coding sequences by multimerizing a 21-bp sequence encompassing the predicted homeodomain binding site sequence, TAATGA, known to be essential in OR gene choice. Singular gene choice is maintained in these "MouSensors." In vivo synaptopHluorin imaging of odor-induced responses by known M71 ligands shows functional glomerular activation in an M71 MouSensor. Moreover, a behavioral avoidance task demonstrates that specific odor detection thresholds are significantly decreased in multiple transgenic lines, expressing mouse or human ORs. We have developed a versatile platform to study gene choice and axon identity, to create biosensors with great translational potential, and to finally decode human olfaction.

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