ATRIP Deacetylation by SIRT2 Drives ATR Checkpoint Activation by Promoting Binding to RPA-ssDNA | Zendy

Hui Zhang | Zendy; PamelaSara E. Head | Zendy; Waaqo Daddacha | Zendy; SeongHoon Park | Zendy; Xingzhe Li | Zendy; Yunfeng Pan | Zendy; Matthew Z. Madden | Zendy; Duc M. Duong | Zendy; Maohua Xie | Zendy; Bing Yu | Zendy; Matthew D. Warren | Zendy; Elaine A. Liu | Zendy; Vishal R. Dhere | Zendy; Chunyang Li | Zendy; Iván Pradilla | Zendy; Mylin A. Torres | Zendy; Ya Wang | Zendy; William S. Dynan | Zendy; Paul W. Doetsch | Zendy; Xingming Deng | Zendy; Nicholas T. Seyfried | Zendy; David Gius | Zendy; David S. Yu | Zendy

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ATRIP Deacetylation by SIRT2 Drives ATR Checkpoint Activation by Promoting Binding to RPA-ssDNA

Author(s) -

Hui Zhang,

PamelaSara E. Head,

Waaqo Daddacha,

SeongHoon Park,

Xingzhe Li,

Yunfeng Pan,

Matthew Z. Madden,

Duc M. Duong,

Maohua Xie,

Bing Yu,

Matthew D. Warren,

Elaine A. Liu,

Vishal R. Dhere,

Chunyang Li,

Iván Pradilla,

Mylin A. Torres,

Ya Wang,

William S. Dynan,

Paul W. Doetsch,

Xingming Deng,

Nicholas T. Seyfried,

David Gius,

David S. Yu

Publication year - 2016

Publication title -

cell reports

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 6.264

H-Index - 154

eISSN - 2639-1856

pISSN - 2211-1247

DOI - 10.1016/j.celrep.2016.01.018

Subject(s) - sirt2 , acetylation , sirtuin , dna replication , dna damage , chek1 , microbiology and biotechnology , g2 m dna damage checkpoint , chemistry , cancer research , biology , dna , biochemistry , cell cycle checkpoint , cell cycle , gene

The ataxia telangiectasia-mutated and Rad3-related (ATR) kinase checkpoint pathway maintains genome integrity; however, the role of the sirtuin 2 (SIRT2) acetylome in regulating this pathway is not clear. We found that deacetylation of ATR-interacting protein (ATRIP), a regulatory partner of ATR, by SIRT2 potentiates the ATR checkpoint. SIRT2 interacts with and deacetylates ATRIP at lysine 32 (K32) in response to replication stress. SIRT2 deacetylation of ATRIP at K32 drives ATR autophosphorylation and signaling and facilitates DNA replication fork progression and recovery of stalled replication forks. K32 deacetylation by SIRT2 further promotes ATRIP accumulation to DNA damage sites and binding to replication protein A-coated single-stranded DNA (RPA-ssDNA). Collectively, these results support a model in which ATRIP deacetylation by SIRT2 promotes ATR-ATRIP binding to RPA-ssDNA to drive ATR activation and thus facilitate recovery from replication stress, outlining a mechanism by which the ATR checkpoint is regulated by SIRT2 through deacetylation.

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