PRC2 Is Required to Maintain Expression of the Maternal Gtl2-Rian-Mirg Locus by Preventing De Novo DNA Methylation in Mouse Embryonic Stem Cells | Zendy

Partha Pratim Das | Zendy; David A. Hendrix | Zendy; Effie Apostolou | Zendy; Alice H. Buchner | Zendy; Matthew C. Canver | Zendy; Semir Beyaz | Zendy; Damir Ljuboja | Zendy; Rachael Kuintzle | Zendy; Woojin Kim | Zendy; Rahul Karnik | Zendy; Zhen Shao | Zendy; Huafeng Xie | Zendy; Jian Xu | Zendy; Alejandro De Los Angeles | Zendy; Yingying Zhang | Zendy; Junho Choe | Zendy; Don Leong Jia Jun | Zendy; Xiaohua Shen | Zendy; Richard I. Gregory | Zendy; George Q. Daley | Zendy; Alexander Meissner | Zendy; Manolis Kellis | Zendy; Konrad Hochedlinger | Zendy; Jonghwan Kim | Zendy; Stuart H. Orkin | Zendy

Open Access

PRC2 Is Required to Maintain Expression of the Maternal Gtl2-Rian-Mirg Locus by Preventing De Novo DNA Methylation in Mouse Embryonic Stem Cells

Author(s) -

Partha Pratim Das,

David A. Hendrix,

Effie Apostolou,

Alice H. Buchner,

Matthew C. Canver,

Semir Beyaz,

Damir Ljuboja,

Rachael Kuintzle,

Woojin Kim,

Rahul Karnik,

Zhen Shao,

Huafeng Xie,

Jian Xu,

Alejandro De Los Angeles,

Yingying Zhang,

Junho Choe,

Don Leong Jia Jun,

Xiaohua Shen,

Richard I. Gregory,

George Q. Daley,

Alexander Meissner,

Manolis Kellis,

Konrad Hochedlinger,

Jonghwan Kim,

Stuart H. Orkin

Publication year - 2015

Publication title -

cell reports

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 6.264

H-Index - 154

eISSN - 2639-1856

pISSN - 2211-1247

DOI - 10.1016/j.celrep.2015.07.053

Subject(s) - prc2 , dna methylation , biology , locus (genetics) , methyltransferase , genetics , methylation , gene , microrna , epigenetics , microbiology and biotechnology , gene expression , ezh2

Polycomb Repressive Complex 2 (PRC2) function and DNA methylation (DNAme) are typically correlated with gene repression. Here, we show that PRC2 is required to maintain expression of maternal microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) from the Gtl2-Rian-Mirg locus, which is essential for full pluripotency of iPSCs. In the absence of PRC2, the entire locus becomes transcriptionally repressed due to gain of DNAme at the intergenic differentially methylated regions (IG-DMRs). Furthermore, we demonstrate that the IG-DMR serves as an enhancer of the maternal Gtl2-Rian-Mirg locus. Further analysis reveals that PRC2 interacts physically with Dnmt3 methyltransferases and reduces recruitment to and subsequent DNAme at the IG-DMR, thereby allowing for proper expression of the maternal Gtl2-Rian-Mirg locus. Our observations are consistent with a mechanism through which PRC2 counteracts the action of Dnmt3 methyltransferases at an imprinted locus required for full pluripotency.

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