The Nuclear PolyA-Binding Protein Nab2p Is Essential for mRNA Production | Zendy

Manfred Schmid | Zendy; Paweł Olszewski | Zendy; Vicent Pelechano | Zendy; Ishaan Gupta | Zendy; Lars M. Steinmetz | Zendy; Torben Heick Jensen | Zendy

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The Nuclear PolyA-Binding Protein Nab2p Is Essential for mRNA Production

Author(s) -

Manfred Schmid,

Paweł Olszewski,

Vicent Pelechano,

Ishaan Gupta,

Lars M. Steinmetz,

Torben Heick Jensen

Publication year - 2015

Publication title -

cell reports

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 6.264

H-Index - 154

eISSN - 2639-1856

pISSN - 2211-1247

DOI - 10.1016/j.celrep.2015.06.008

Subject(s) - polyadenylation , messenger rna , rna binding protein , microbiology and biotechnology , biology , rna , biogenesis , nuclear protein , gene expression , precursor mrna , transcription (linguistics) , genetics , gene , rna splicing , transcription factor , linguistics , philosophy

Polyadenylation of mRNA is a key step in eukaryotic gene expression. However, despite the major impact of poly(A) tails on mRNA metabolism, the precise roles of poly(A)-binding proteins (PABPs) in nuclear mRNA biogenesis remain elusive. Here, we demonstrate that rapid nuclear depletion of the S. cerevisiae PABP Nab2p leads to a global loss of cellular mRNA, but not of RNA lacking poly(A) tails. Disappearance of mRNA is a nuclear event, but not due to decreased transcription. Instead, the absence of Nab2p results in robust nuclear mRNA decay by the ribonucleolytic RNA exosome in a polyadenylation-dependent process. We conclude that Nab2p is required to protect early mRNA and therefore constitutes a crucial nuclear mRNA biogenesis factor.

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