Nontemplated Nucleotide Additions Distinguish the Small RNA Composition in Cells from Exosomes | Zendy

Danijela KoppersLalic | Zendy; Michael Hackenberg | Zendy; Irene V. Bijnsdorp | Zendy; Monique A.J. van Eijndhoven | Zendy; Payman Sadek | Zendy; Daoud Sie | Zendy; Nicoletta Zini | Zendy; Jaap M. Middeldorp | Zendy; Bauke Ylstra | Zendy; Renée X. de Menezes | Zendy; Thomas Würdinger | Zendy; Gerrit A. Meijer | Zendy; D. Michiel Pegtel | Zendy

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Nontemplated Nucleotide Additions Distinguish the Small RNA Composition in Cells from Exosomes

Author(s) -

Danijela KoppersLalic,

Michael Hackenberg,

Irene V. Bijnsdorp,

Monique A.J. van Eijndhoven,

Payman Sadek,

Daoud Sie,

Nicoletta Zini,

Jaap M. Middeldorp,

Bauke Ylstra,

Renée X. de Menezes,

Thomas Würdinger,

Gerrit A. Meijer,

D. Michiel Pegtel

Publication year - 2014

Publication title -

cell reports

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 6.264

H-Index - 154

eISSN - 2639-1856

pISSN - 2211-1247

DOI - 10.1016/j.celrep.2014.08.027

Subject(s) - microvesicles , rna , microrna , biology , microbiology and biotechnology , small rna , exosome , extracellular vesicles , non coding rna , computational biology , gene , genetics

Functional biomolecules, including small noncoding RNAs (ncRNAs), are released and transmitted between mammalian cells via extracellular vesicles (EVs), including endosome-derived exosomes. The small RNA composition in cells differs from exosomes, but underlying mechanisms have not been established. We generated small RNA profiles by RNA sequencing (RNA-seq) from a panel of human B cells and their secreted exosomes. A comprehensive bioinformatics and statistical analysis revealed nonrandomly distributed subsets of microRNA (miRNA) species between B cells and exosomes. Unexpectedly, 3' end adenylated miRNAs are relatively enriched in cells, whereas 3' end uridylated isoforms appear overrepresented in exosomes, as validated in naturally occurring EVs isolated from human urine samples. Collectively, our findings suggest that posttranscriptional modifications, notably 3' end adenylation and uridylation, exert opposing effects that may contribute, at least in part, to direct ncRNA sorting into EVs.

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