Origin-Dependent Neural Cell Identities in Differentiated Human iPSCs In Vitro and after Transplantation into the Mouse Brain | Zendy

Gunnar Hargus | Zendy; Marc Ehrlich | Zendy; Marcos J. AraúzoBravo | Zendy; Kathrin Hemmer | Zendy; Anna-Lena Hallmann | Zendy; Peter Reinhardt | Zendy; Kee-Pyo Kim | Zendy; Kenjiro Adachi | Zendy; Simeon Santourlidis | Zendy; Foued Ghanjati | Zendy; Mareike Fauser | Zendy; Christiana Ossig | Zendy; Alexander Storch | Zendy; Jeong Beom Kim | Zendy; Jens C. Schwamborn | Zendy; Jared Sterneckert | Zendy; Hans R. Schöler | Zendy; Tanja Kuhlmann | Zendy; Holm Zaehres | Zendy

Open Access

Origin-Dependent Neural Cell Identities in Differentiated Human iPSCs In Vitro and after Transplantation into the Mouse Brain

Author(s) -

Gunnar Hargus,

Marc Ehrlich,

Marcos J. AraúzoBravo,

Kathrin Hemmer,

Anna-Lena Hallmann,

Peter Reinhardt,

Kee-Pyo Kim,

Kenjiro Adachi,

Simeon Santourlidis,

Foued Ghanjati,

Mareike Fauser,

Christiana Ossig,

Alexander Storch,

Jeong Beom Kim,

Jens C. Schwamborn,

Jared Sterneckert,

Hans R. Schöler,

Tanja Kuhlmann,

Holm Zaehres

Publication year - 2014

Publication title -

cell reports

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 6.264

H-Index - 154

eISSN - 2639-1856

pISSN - 2211-1247

DOI - 10.1016/j.celrep.2014.08.014

Subject(s) - neuroectoderm , induced pluripotent stem cell , biology , neural stem cell , transplantation , microbiology and biotechnology , mesoderm , progenitor cell , neural development , stem cell , embryonic stem cell , genetics , gene , medicine

The differentiation capability of induced pluripotent stem cells (iPSCs) toward certain cell types for disease modeling and drug screening assays might be influenced by their somatic cell of origin. Here, we have compared the neural induction of human iPSCs generated from fetal neural stem cells (fNSCs), dermal fibroblasts, or cord blood CD34(+) hematopoietic progenitor cells. Neural progenitor cells (NPCs) and neurons could be generated at similar efficiencies from all iPSCs. Transcriptomics analysis of the whole genome and of neural genes revealed a separation of neuroectoderm-derived iPSC-NPCs from mesoderm-derived iPSC-NPCs. Furthermore, we found genes that were similarly expressed in fNSCs and neuroectoderm, but not in mesoderm-derived iPSC-NPCs. Notably, these neural signatures were retained after transplantation into the cortex of mice and paralleled with increased survival of neuroectoderm-derived cells in vivo. These results indicate distinct origin-dependent neural cell identities in differentiated human iPSCs both in vitro and in vivo.

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