A Chemical Probe that Labels Human Pluripotent Stem Cells | Zendy

Nao Hirata | Zendy; Masato Nakagawa | Zendy; Yuto Fujibayashi | Zendy; Kaori Yamauchi | Zendy; Asako Murata | Zendy; Itsunari Minami | Zendy; Maiko Tomioka | Zendy; Takayuki Kondo | Zendy; TingFang Kuo | Zendy; Hiroshi Endô | Zendy; Haruhisa Inoue | Zendy; Shinichi Sato | Zendy; Shin Ando | Zendy; Yoshinori Kawazoe | Zendy; Kazuhiro Aiba | Zendy; Koh Nagata | Zendy; Eihachiro Kawase | Zendy; YoungTae Chang | Zendy; Hirofumi Suemori | Zendy; Koji Eto | Zendy; Hiromitsu Nakauchi | Zendy; Shinya Yamanaka | Zendy; Norio Nakatsuji | Zendy; Kazumitsu Ueda | Zendy; Motonari Uesugi | Zendy

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A Chemical Probe that Labels Human Pluripotent Stem Cells

Author(s) -

Nao Hirata,

Masato Nakagawa,

Yuto Fujibayashi,

Kaori Yamauchi,

Asako Murata,

Itsunari Minami,

Maiko Tomioka,

Takayuki Kondo,

TingFang Kuo,

Hiroshi Endô,

Haruhisa Inoue,

Shinichi Sato,

Shin Ando,

Yoshinori Kawazoe,

Kazuhiro Aiba,

Koh Nagata,

Eihachiro Kawase,

YoungTae Chang,

Hirofumi Suemori,

Koji Eto,

Hiromitsu Nakauchi,

Shinya Yamanaka,

Norio Nakatsuji,

Kazumitsu Ueda,

Motonari Uesugi

Publication year - 2014

Publication title -

cell reports

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 6.264

H-Index - 154

eISSN - 2639-1856

pISSN - 2211-1247

DOI - 10.1016/j.celrep.2014.02.006

Subject(s) - induced pluripotent stem cell , stem cell , microbiology and biotechnology , biology , human induced pluripotent stem cells , embryonic stem cell , chemistry , biochemistry , gene

A small-molecule fluorescent probe specific for human pluripotent stem cells would serve as a useful tool for basic cell biology research and stem cell therapy. Screening of fluorescent chemical libraries with human induced pluripotent stem cells (iPSCs) and subsequent evaluation of hit molecules identified a fluorescent compound (Kyoto probe 1 [KP-1]) that selectively labels human pluripotent stem cells. Our analyses indicated that the selectivity results primarily from a distinct expression pattern of ABC transporters in human pluripotent stem cells and from the transporter selectivity of KP-1. Expression of ABCB1 (MDR1) and ABCG2 (BCRP), both of which cause the efflux of KP-1, is repressed in human pluripotent stem cells. Although KP-1, like other pluripotent markers, is not absolutely specific for pluripotent stem cells, the identified chemical probe may be used in conjunction with other reagents.

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