Specific miRNA Stabilization by Gld2-Catalyzed Monoadenylation | Zendy

Andrea D’Ambrogio | Zendy; Weifeng Gu | Zendy; Tsuyoshi Udagawa | Zendy; Craig C. Mello | Zendy; Joel D. Richter | Zendy

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Specific miRNA Stabilization by Gld2-Catalyzed Monoadenylation

Author(s) -

Andrea D’Ambrogio,

Weifeng Gu,

Tsuyoshi Udagawa,

Craig C. Mello,

Joel D. Richter

Publication year - 2012

Publication title -

cell reports

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 6.264

H-Index - 154

eISSN - 2639-1856

pISSN - 2211-1247

DOI - 10.1016/j.celrep.2012.10.023

Subject(s) - microrna , translation (biology) , transcription (linguistics) , biology , messenger rna , microbiology and biotechnology , nucleotide , regulation of gene expression , gene , genetics , linguistics , philosophy

MicroRNAs (miRNAs) are small, noncoding RNAs that inhibit translation and promote mRNA decay. The levels of mature miRNAs are the result of different rates of transcription, processing, and turnover. The noncanonical polymerase Gld2 has been implicated in the stabilization of miR-122, possibly through catalyzing 3' monoadenylation; however, there is little evidence that this relationship is one of cause and effect. Here, we biochemically characterize Gld2's involvement in miRNA monoadenylation and its effect on miRNA stability. We find that Gld2 directly monoadenylates and stabilizes specific miRNA populations in human fibroblasts and that sensitivity to monoadenylation-induced stability depends on nucleotides in the miRNA 3' end. These results establish a mechanism of miRNA stability and resulting posttranscriptional gene regulation.

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