Up-regulation of Syndecan-4 contributes to TGF-β1-induced epithelial to mesenchymal transition in lung adenocarcinoma A549 cells | Zendy

Yoko Toba-Ichihashi | Zendy; Toshimitsu Yamaoka | Zendy; Tohru Ohmori | Zendy; Motoi Ohba | Zendy

Open Access

Up-regulation of Syndecan-4 contributes to TGF-β1-induced epithelial to mesenchymal transition in lung adenocarcinoma A549 cells

Author(s) -

Yoko Toba-Ichihashi,

Toshimitsu Yamaoka,

Tohru Ohmori,

Motoi Ohba

Publication year - 2015

Publication title -

biochemistry and biophysics reports

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.641

H-Index - 23

ISSN - 2405-5808

DOI - 10.1016/j.bbrep.2015.11.021

Subject(s) - epithelial–mesenchymal transition , gene knockdown , syndecan 1 , microbiology and biotechnology , vimentin , a549 cell , slug , chemistry , transforming growth factor , cell , downregulation and upregulation , cancer research , biology , immunology , biochemistry , apoptosis , immunohistochemistry , gene

yndecan-4 (SDC4) is a cell-surface proteoglycan associated with cell adhesion, motility, and intracellular signaling. Here, we present that SDC4 functions as a positive regulator of the transforming growth factor (TGF)-β1-induced epithelial to mesenchymal transition (EMT) via Snail in lung adenocarcinoma, A549 cells. TGF-β1 up-regulated the expression of SDC4, accompanied by the induction of EMT. Wound-healing and transwell chemotaxis assay revealed that SDC4 promoted cell migration and invasion. SDC4 knockdown recovered the E-cadherin and decreased vimentin and Snail expression in EMT-induced A549 cells. However, depletion of SDC4 resulted in little change of the Slug protein expression and mesenchymal cell morphology induced by TGF-β1. The double knockdown of SDC-4 and Slug was required for reversal of epithelial morphology; it did not occur from the SDC4 single knockdown. These findings suggest that Snail is a transcriptional factor downstream of SDC4, and SDC4 regulates TGF-β1-induced EMT by cooperating with Slug. Our data provide a novel insight into cellular mechanisms, whereby the cell-surface proteoglycan modulated TGF-β1-induced EMT in lung adenocarcinoma, A549 cells

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